Influence of successive and combined ultraviolet A and B irradiations on matrix metalloelastases produced by human dermal fibroblasts in culture.

Author: Kut C, Hornebeck W, Groult N, Redziniack G, Godeau G, Pellat B

Author affiliation: Biochemistry Department, Faculty of Dental Surgery, University Rene Descartes Paris V, 1 rue M. Arnoux, Montrouge, 92120, France.

Publication date & source: 1997.06, Cell Biol Int., 21(6):347-52.

To study the cumulative influence of UV irradiations on skin matrix alterations, human skin fibroblasts were irradiated successively three-fold, at 24 h intervals, with UVA (3x5J/cm2), UVB (3x8mJ/cm2), UVA plus UVB (3x5J/cm2 and 3x8mJ/cm2) and the levels of 92 kDa gelatinase (pro-MMP9), 72 kDa gelatinase (pro-MMP2) and plasma-membrane elastase type protease were determined, following subsequent 24-h culture in 10% serum-containing medium. UV irradiations had only minor influence (1.4-fold increase for UVB) on secreted levels of pro-MMP2 and decreased the amount of plasma membrane elastase produced by cells. It did however, for UVA and UVB alone, induce a significant increase of 66 kDa activated MMP2 production: 2.5- and 1.7-fold respectively. Such enhancement was not observed when combined irradiations were administered. UV exposure possessed a much higher influence on pro-MMP9 secretion by dermal fibroblast enhancing enzyme levels by 2.5-, 6.5- and 5-fold for UVA, UVB and UVA+UVB, respectively.

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